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M9480107.TXT
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1994-08-09
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Document 0107
DOCN M9480107
TI Surface expression of the HIV-1 envelope proteins in env
gene-transfected CD4-positive human T cell clones: characterization and
killing by an antibody-dependent cellular cytotoxic mechanism.
DT 9410
AU Ahmad A; Yao XA; Tanner JE; Cohen E; Menezes J; Laboratory of
Immunovirology, University of Montreal, Quebec,; Canada.
SO J Acquir Immune Defic Syndr. 1994 Aug;7(8):789-98. Unique Identifier :
AIDSLINE MED/94293160
AB The env gene of the human immunodeficiency virus-type 1 (HIV-1) was
transfected in CEM-nkr, a human lymphoid cell line of T lineage that is
resistant to the activity of natural killer cells, and for the first
time, transfected T cell clones were established that stably express
gp160 intracellularly and gp120 on the surface as demonstrated by
radioimmunoprecipitation as well as by indirect membrane
immunofluorescence. The regulatory protein vpu was not detected by
radioimmunoprecipitation in these clones. The surface expression of
gp120 without vpu in these clones provides direct evidence that gp160 is
processed and cleaved (without vpu) in CD4+ cells. The CD4 antigens of
these cells coprecipitated gp160; interestingly, no reduction of the
surface CD4 expression (detectable by flow cytometric analysis of
membrane immunofluorescence with OKT4) in the transfected cells was
observed. However, decreased reactivity of the transfected clones with
OKT4A was observed. The gp120-expressing cells did not form syncytia on
coculture with other CD4+ human cell lines. These observations suggest
the binding of gp120 to the surface CD4 antigen of the transfected
cells. The transfected cells retained their resistance to the activity
of the natural killer cells but showed a significant (p < 0.05) lysis
when they were preincubated with AIDS patients' serum containing
anti-gp120/41 antibodies. Thus, the expressed gp120/41 in these cells
made them susceptible to killing by an antibody-dependent cellular
cytotoxicity (ADCC) mechanism. To our knowledge, these are the first
reported CD4+ T cell lines that stably express HIV envelope proteins.
These cell lines would be useful as targets in exploring
gp120/41-specific immune responses, especially in conducting
gp120/41-specific ADCC studies in HIV-infected or gp120/41
(gp160)-vaccinated individuals.
DE *Antibody-Dependent Cell Cytotoxicity Antigens, CD4/IMMUNOLOGY Cell
Line Clone Cells Fluorescent Antibody Technique Gene Expression
Regulation, Viral Gene Products, env/*BIOSYNTHESIS/GENETICS/IMMUNOLOGY
*Genes, env Giant Cells Human HIV Envelope Protein
gp120/BIOSYNTHESIS/GENETICS/IMMUNOLOGY HIV-1/GENETICS/*IMMUNOLOGY
Killer Cells, Natural/IMMUNOLOGY Protein
Precursors/BIOSYNTHESIS/GENETICS/IMMUNOLOGY Radioimmunoprecipitation
Assay Support, Non-U.S. Gov't Transfection T4
Lymphocytes/*MICROBIOLOGY JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).